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Expressed Sequence Tags Analysis and Design of Simple Sequence Repeats Markers from a Full-Length cDNA Library in Perilla frutescens (L.)

Identifieur interne : 000649 ( Main/Exploration ); précédent : 000648; suivant : 000650

Expressed Sequence Tags Analysis and Design of Simple Sequence Repeats Markers from a Full-Length cDNA Library in Perilla frutescens (L.)

Auteurs : Eun Soo Seong [Corée du Sud] ; Ji Hye Yoo [Corée du Sud] ; Jae Hoo Choi [Corée du Sud] ; Chang Heum Kim [Corée du Sud] ; Mi Ran Jeon [Corée du Sud] ; Byeong Ju Kang [Corée du Sud] ; Jae Geun Lee [Corée du Sud] ; Seon Kang Choi [Corée du Sud] ; Bimal Kumar Ghimire [Corée du Sud] ; Chang Yeon Yu [Corée du Sud]

Source :

RBID : PMC:4668317

Abstract

Perilla frutescens is valuable as a medicinal plant as well as a natural medicine and functional food. However, comparative genomics analyses of P. frutescens are limited due to a lack of gene annotations and characterization. A full-length cDNA library from P. frutescens leaves was constructed to identify functional gene clusters and probable EST-SSR markers via analysis of 1,056 expressed sequence tags. Unigene assembly was performed using basic local alignment search tool (BLAST) homology searches and annotated Gene Ontology (GO). A total of 18 simple sequence repeats (SSRs) were designed as primer pairs. This study is the first to report comparative genomics and EST-SSR markers from P. frutescens will help gene discovery and provide an important source for functional genomics and molecular genetic research in this interesting medicinal plant.


Url:
DOI: 10.1155/2015/679548
PubMed: 26664999
PubMed Central: 4668317


Affiliations:


Links toward previous steps (curation, corpus...)


Le document en format XML

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<p>
<italic>Perilla frutescens</italic>
is valuable as a medicinal plant as well as a natural medicine and functional food. However, comparative genomics analyses of
<italic>P. frutescens</italic>
are limited due to a lack of gene annotations and characterization. A full-length cDNA library from
<italic>P. frutescens</italic>
leaves was constructed to identify functional gene clusters and probable EST-SSR markers via analysis of 1,056 expressed sequence tags. Unigene assembly was performed using basic local alignment search tool (BLAST) homology searches and annotated Gene Ontology (GO). A total of 18 simple sequence repeats (SSRs) were designed as primer pairs. This study is the first to report comparative genomics and EST-SSR markers from
<italic>P. frutescens</italic>
will help gene discovery and provide an important source for functional genomics and molecular genetic research in this interesting medicinal plant.</p>
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</TEI>
<affiliations>
<list>
<country>
<li>Corée du Sud</li>
</country>
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<li>Séoul</li>
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</tree>
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</record>

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